Hi Michele,
Please follow the instructions on the GUI. --ctf means that
CTF-correction will be done inside the new refinement, so the output
references from that one will be CTF-corrected.
HTH,
Sjors
On 10/12/2016 11:39 AM, Michele Lunelli wrote:
> Dear all,
>
> I am confused about the CTF correction of the references in relion_autopick
> and relion_particle_sort (relion 1.4).
> My references are already CTF-corrected, because they are classes from a 2D
> classification done with relion, or projections from a previous relion
> reconstruction, generated with relion_project. Therefore I set "Yes" in the
> GUI question "Are References CTF corrected?". Then the --ctf option is
> added to the command line arguments when the two programs are run. However,
> the command line help of the two programs states:
> --ctf (false) : Perform CTF correction on the references?
> which I undestand as: Do the CTF correction of the references if the --ctf
> option is specified.
>
> Which one is the correct interpretation, the GUI or the command line?
> Should I specify the --ctf option if my references are already
> CTF-corrected?
>
>
> Thank you in advance
> Michele
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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