If Wayne is even close on the size of your direct dimension, Ičll second
his suggestion to trim it down, in many bioNMR experiments signal decays
in the first 600-800 complex points or so of the FID anyway. 2k points
usually gives enough resolution for most anything I need, occasionally
Ičll extend a 2D to 4k. Also, if youčre not converting to a blocking data
format like Justin mentioned, youčll have problems. With 2007-vintage
computers, larger 3D datasets become painful if kept in NMRPipe format
(and almost unusable if you want to display XZ or YZ instead of XY).
Ičd strongly suggest that once you find a reasonable contour level for
your 4D NOESY and then let Analysis create contour files for the spectrum:
Experiment->Spectra, choose the Display Options tab, select you spectrum,
click Contour Files, in the new window click Create New File, choose your
4D and the two dimensions you plan to view most of the time, then click
Contour and Save. This should greatly speed up scrolling and redrawing
since otherwise the software calculated the contours on the fly, although
if you want to adjust contours at a particular place youčll need to toggle
using the contour file on and off in the Display Options tab. If youčll be
looking at an orthogonal view more than occasionally, youčll need to make
contour files for each pair of two dimensions you plan to look at. For a
large 3D creating a contour file can take several minutes, so Ičd start
contouring before you go home for the day. The files will be large.
Also, avoid scrolling as much as possible. Since youčre looking at a
13C,15N edited NOESY, two dimensions will be the same as your 15N HSQC.
View the other two dimensions of your 4D and go through one HSQC peak at a
time.
As a last resort if the data is still painful to work with, you can do
what I had to do with a large (>2 GB) 3D 13C NOESY under 32-bit Analysis
and break your 4D up into multiple spectra. Exactly where will be up to
both your personal preference and the data. Ičd suggest about 4-8 chunks.
In my case, I processed the data with NMRPipe twice and extracted
different 13C regions (aliphatics vs. aromatics, and threw away about 30
ppm of 13C with no signals). Keep in mind that as far as Analysis is
concerned, these will all be separate spectra and youčll have to combine
the peak lists later.
Andrew
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