Hi,
I encountered a problem when I try to do particle sorting after I have done Particle extraction of autopicking result. The following is the my setting for particle sorting. I saved selected good classes with four from eight in a star file called best_classes.star.
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Executing: mpirun -n 6 `which relion_particle_sort_mpi` --i particles_autopick.star --ref best_classes.star --angpix 1.336 --particle_diameter 600 --ctf &
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The error that was reported:
Calculating sorting features for all input particles...
000/??? sec ~~(,_,"> [o2.08/25.07 min ....~~(,_,">ParticleSorter::calculateFeatures: cannot find class number in input STAR file
File: src/particle_sorter.cpp line: 262
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Sorry! You were supposed to get help about:
mpi-abort
But I couldn't open the help file:
/net/xychen/xychen12/usr_test/x86_64/share/openmpi/help-mpi-api.txt: No such file or directory. Sorry!
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It seems that it can't find class number in my best_classes.star file. However, I can find the class number column in my best_classes.star file.
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best_classes.star
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data_
loop_
_rlnAccuracyRotations #1
_rlnAccuracyTranslations #2
_rlnClassDistribution #3
_rlnClassPriorOffsetX #4
_rlnClassPriorOffsetY #5
_rlnReferenceImage #6
_rlnClassNumber #7
1.080000 1.760000 0.364610 1.967450 2.389999 [log in to unmask] 1
1.800000 1.450000 0.296108 3.408169 3.197371 [log in to unmask] 3
0.970000 1.920000 0.159751 2.088517 1.683450 [log in to unmask] 4
0.600000 1.540000 0.061028 1.341150 0.789700 [log in to unmask] 7
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May I ask if you can give me some guidance?
Thanks in advance.
Best regards,
Chen
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