any clean light oil is ok, I used paraffin oil, the same that is used to overlay PCR reactions (or was, before “hot-lid” PCR machines became common).
Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://wwwuser.cnb.csic.es/~mjvanraaij
> On 4 Aug 2016, at 03:10, 张士军 <[log in to unmask]> wrote:
>
> Dear Mesters
> Thanks for your advice ! I had try diffract my crystals in room temperature ,and the diffraction spots are not smearing ,just the resolution problem.Does that mean ,the smearing problem is because of unsuitable cryobuffer not the crystal itself ?And what kind of oil do you suggest ?Is it paratone from Hampton OK?Thanks a lot !
> yours
> shijun
> 在2016-07-26 14:53:38,张士军[log in to unmask]写道:
>
> It is because of evaporation of the volatile component and this is not good for your crystals at all.
>
> Try one of the following things:
> 1) Try to grow and loop the crystals in the cold room
> 2) Cover the drop with oil immediately after cutting the tape
> 3) Grow the crystals in a gel or a thin capillary
>
> J.
>
> Am 25.07.16 um 13:22 schrieb SUBSCRIBE CCP4BB ShijunZhang:
>> DEAR Mark J van Raaij
>> when i open the tape of some sitting drop,the crystals are rotating,and it takes me long time to loop it
>>
>
> --
> Dr. math. et dis. nat. Jeroen R. Mesters
> Deputy, Senior Researcher & Lecturer
> Program Coordinator Infection Biology
>
> Institute of Biochemistry, University of Lübeck
> Ratzeburger Allee 160, 23538 Lübeck, Germany
> phone: +49-451-31013105 (secretariate -31013101)
> fax: +49-451-31013104
>
>
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