HI Shijun,
Dunking directly into liquid nitrogen also works. Be sure to limit the transfer distance from vial to nitrogen. After equilibration, for mounting we usually set the vial on the table next to the liquid nitrogen dewar. Carefully apply the wand to the crystal cap and then transfer in one quick motion.
Doug
> On Aug 17, 2016, at 12:29 AM, 张士军 <[log in to unmask]> wrote:
>
> Dear Doug Juers
> Thanks for your advice . I have read that paper ,but they mounted in the diffractometer from vial directly after equilibrating .I wonder whether could I put in the liquid nitrogen ,and transport to sychron in my country ?Thanks a lot
> shijun
>
> 在2016-08-17 07:51:33,张士军<[log in to unmask]>写道:
>> Hello Shijun,
>>
>> I also just saw your post. Your well solution of 30% isopropanol with PEG 1000 may be a good cryoprotective agent. If you are able to loop a crystal, you might try placing the crystalcap with looped crystal in a cryovial containing 500 microliters of your well solution. Plug the liquid nitrogen escape holes with a little clay. The crystal will then move back towards vapor equilibration with the original well solution. Let the crystal recover for a couple minutes (back up to full isopropanol concentration) before transferring the crystalcap rapidly to liquid nitrogen. If you get ice rings, then increase the isopropanol concentration in the cryovial.
>>
>> http://www.ncbi.nlm.nih.gov/pubmed/25286441
>>
>>
>> -Doug
>>
>> Douglas Juers
>> Associate Professor
>> Physics Department
>> Program in BBMB, Director
>> Whitman College
>> Walla Walla, WA 99362
>> Phone: 509-527-5229 FAX: 509-527-5904
>> Web: http://www.whitman.edu/~juersdh/
>>
>>
>
>
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