Dear Rubbiya,
You're using a 'particle mask diameter (in A)' (on the General job-type)
that is larger than the edge of your box (box size in pixels is on
'extract' tab of the Extraction job-type; pixel size in Angstroms is on
the General job-type). This is not allowed.
HTH,
Sjors
On 07/18/2016 06:46 PM, Rubbiya Ali wrote:
> Hi everyone,
>
> This is first time I am processing my own dataset using Relion, but getting following error at particle extraction step:
>
> "normalise error: 2*bg_radius is larger than image size! at particle extraction step."
>
> I checked many things but not sure why it's not getting fixed. Can some one help me with it?
> Your any kind of help will be highly appreciated.
>
> Regards,
> Rubbiya Ali
> PhD I Hankamer Group I
> Institute for Molecular Bioscience I Queensland Bioscience Precinct I
> The University of Queensland I Brisbane Queensland 4072 I Australia
> Tel: +61 7 334 62015
> Email: [log in to unmask]
> Skype: rubbiya.ali
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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