Hi Yashar,
There is an all-zero particle in your images. Perhaps your procedures
led you to pick one outside a micrograph? You could run the following
command to find it:
relion_image_handler --stats --i [starfile]
HTH,
Sjors
On 07/15/2016 04:53 PM, Yashar Sadian wrote:
> Hi
> I am trying to extract the movie particles but I receive the following error:
>
>
> WARNING! Stddev of image Particles/../micrographs/242-166_003_ali_movie_particles_smallbox.mrcs is zero! Skipping normalisation...
>
>
> I acquired tif and mrc on the microscope and used imod clip and motioncorr for binning and correction. However I did the processing on the mrc files from the microscope.
>
>
> Thanks
> Yashar
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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