Hi Tat,
If your data has been phase-flipped, you should always use
--ctf_phase_flipped. You still have the choice to ignore CTFs until their
first peak.
HTH,
Sjors
> Hi all,
>
> I have been trying to do some classification on an old dataset that has
> been phase-flipped only but not fully ctf-corrected. I realised there is
> an option in RELION --ctf_phase_flipped, does it apply only to the data
> that has been ctf-corrected? or is it also applicable to phase flipped
> only data? I tried some classifications with this flag on, but the class
> distributions were not converging well at all.
>
> Thanks a lot.
>
> Tat
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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