Hello everyone,
I have some questions for my VBM analysis result. I compared the
brain structure of Chinese dyslexia children (DD), age-matched
controls(AC) and reading-matched controls (RC), and found that one of
overlapped grey matter clusters of DD>AC and DD>RC (30, 0, -2) had
almost the same location as the overlapped white matter cluster of
AC>DD and RC>DD (30, -6,-5). I?m not sure why it happened, and I also
don?t know how to report this result, because I can?t decide whether
this cluster belongs to grey or white matter. I list all of steps I
did during the analysis, could you help me to check it?
1.Using TOM8 to create TPM template based on the gender and age for my
subjects. They are all Chinese children from 8-11 years old.
2.The VBM8 workflow:
1)Normalized and Segmented:
VBM8 ? Estimate and Write
Parameters:
Volumes<-X?Select Files?[select raw data]?Done
Estimation options:
Tissue Probability Map?[use the template made by TOM8 ]
Gaussians per class?[use default]
Bias regularisation?[use default]
Bias FWHM?[use default]
Affine Regularisation?[*ICBM space template-Asian brains]
Warping Regularisation?[use default]
Sample distance?[use default]
Extended options:
Dartel Template?[use default]
Use SANLM de-noising filter?[use default]
MRF Weighting?[use default]
Clean up any partitions?[Light Cleanup]
Display and print results?[use default]
Writing options?[use default]
File?Save Batch
File?Run Batch
2)Check data
VBM8 ?Check data quality?Display one slice for all images
Parameters:
Volumes<-X?Select Files?[select the new files]?Done
Proportional scaling?[yes]
Show slice in mm?[use default]
File?Save Batch
File?Run Batch
VBM8 ?Check data quality?Check Sample Homogeneity Using Covariance
Parameters:
Volumes<-X?Select Files?[select the ?m0wrp1? files]?Done
Proportional scaling?[yes]
Show slice in mm?[0]
Gap to skip slices?[1]
Nuisance?[Enter nuisance variables]
File?Save Batch
File?Run Batch
3)Smooth
SPM menu?Smooth
Parameters:
Images to smooth<-X?Select Files?[select the ?m0wrp1?/?m0wrp2? files]?Done
FWHM?[8,8,8]
Data type?[use defaults]
Filename Prefix?[use defaults]
File?Save Batch
File?Run Batch
3.Group comparison
I don?t know whether the pre-processing causes the mixed result of
grey matter and white matter analysis. Should I change the TPM
template and ICBM template to the default choice? Or should I just
treat it as some characteristics of Chinese dyslexia children? But how
could I report this result in the paper?
I?m looking forward for your reply. Thank you for your help!
Yours,
Zhao Wang
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