Hi Jarrod,
Creating a pseudo-precession image with
labelit.precession_photo would be informative.
The program is described in Nick Sauter's article in
Computational Crystallography Newsletter 2011 Jan.
My program called "dials.rs_mapper", which is included in DIALS,
can reconstruct the reciprocal space and outputs in CCP4 map format.
Then you can volume-render the output in PyMOL.
Best regards,
Takanori Nakane
Am 2016年04月19日 um 17:56 schrieb John R Helliwell:
> Hi,
> Just to also mention for you to check your successive diffraction images
> through the whole crystal sample rotation.
> Thus far I note the general absence of diffuse streaking in the example
> diffraction images, which is at least a 'simplification'.
> Best wishes,
> John
>
> On Mon, Apr 18, 2016 at 4:18 PM, Jarrod Mousa <[log in to unmask]
> <mailto:[log in to unmask]>> wrote:
>
> Dear community,
>
> I collected data from a crystal grown in 30% PEG 8000, 200 mM
> ammonium sulfate, 100 mM sodium cacodylate pH 6.4 and 40% v/v
> Pentaerythritol ethoxylate (3/4 EO/OH), and cryoprotected with 20%
> glycerol. I am attaching a snapshot of the diffraction pattern along
> with a zoomed in portion. I am having trouble solving by molecular
> replacement, even though I have a very good starting model. Just
> wanted to get people's insights into the odd (at least to me)
> diffraction pattern. The data can process with XDS in P 4 2 2. I
> also attached merging statistics.
>
> Thank you,
>
> Jarrod Mousa
> Post-doctoral research fellow
> Crowe Laboratory
> Vanderbilt University Medical Center
>
>
>
>
> --
> Professor John R Helliwell DSc
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