From: | | [log in to unmask][log in to unmask], 8 Apr 2016 15:26:01 +0200547_utf-8 There is clearly not enough information here, but let me attempt an answer, that might be of general interest.
To calculate the IC50 it is not strictly needed to to go down to 100% inhibition. The IC50 is by definition the inhibitor concentration where you achieve 50% activity, provided you corrected for the background (no enzyme), and that you measured the initial velocity properly (choosing a part where the velocity is linear over time, and calculating the slope, as end point assays are typically not enough). [...][log in to unmask] |