Hello all,
I have a question or would like some opinions on doing a broad crystallization screen with micro seeding. We have had good luck over the years using the technique but we usually use microseeds of the protein of interest. They just generally are not good crystals for various reasons. One of the grad students I have been working with has been able to produce amorphous spheres but nothing else. We have tried all sorts of thing over the last few of years and they want to graduate so this is a last gasp effort for them. They will graduate if no crystals are formed but it would be nice to at least have a crystal and possibly a preliminary structure for them to present and later publish.
My question is should we microseed by grinding up the spheres or should we use crystal of a similar protein but from a different organism. There are significant differences in the length and sequence of the two proteins. I originally was going to go with the related protein but then got thinking about the spheres we can get and thought they might also make a good scaffold for crystallization.
Cheers,
Leonard M. Thomas Ph.D.
Macromolecular Crystallography Laboratory
Price Family Foundation Institute of Structural Biology
Department of Chemistry and Biochemistry
University of Oklahoma
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019
405-325-1126
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http://barlywine.chem.ou.edu
http://structuralbiology.ou.edu
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