Hi Brian,
I tried using the "Assignment -> Copy Assignments to transfer the assignments with Consider aliased ticked”. The assignments are transferred/copied for the non-folded peaks, but they are not copied for the folded peaks. The Consider aliased opinion is ticked, and I tried both Assign All Singly Matched and Assign All To Closest. Any ideas?
Best, Mark
> On Mar 3, 2016, at 1:25 AM, Brian Smith <[log in to unmask]> wrote:
>
> Hi Mark,
>
> For the most streamlined way, start by peak picking your non-folded HSQC and use Assignment -> Initialise Root Resonances to assign spin systems to them. Then peak pick your folded spectrum and use Assignment -> Copy Assignments to transfer the assignments with Consider aliased ticked.
>
> Even if you don't do it this way, it's relatively easy to sort things out later with the peak unalias functionality and merging spin systems and resonances.
>
> Brian
>
> Dr. Brian O. Smith --------------------------- Brian Smith at glasgow ac uk
> Institute of Molecular, Cell and Systems Biology & School of Life Sciences,
> College of Medical, Veterinary & Life Sciences,
> Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
> Tel: 0141 330 5167/6459/3089
> ----------------------------------------------------------------------
> The University of Glasgow, charity number SC004401
>
> ________________________________________
> From: CcpNmr software mailing list [[log in to unmask]] on behalf of Kelly, Mark [[log in to unmask]]
> Sent: 02 March 2016 22:32
> To: [log in to unmask]
> Subject: Folded Peaks in Indirect 15N Dimensions
>
> Hello,
>
> I’m working on a small protein and it made sense to fold peaks in the indirect 15N dimensions (2D, 3D). I have an NHSQC which does not have folded peaks and then one with a narrower 15N SW where I have ‘folded’ peaks. How should I best peak the peaks and set-up the project so CCPN will handle the ‘true’ 15N shifts of the folded peaks correctly?
>
> Thanks, Mark
>
>
|