Dear all
I am working on a 2-A structure with an octameric arrangement (363 residues per chain). There are only about 20 water molecules in total, which does not correspond to the rule-of-thumb of one water per residue (Carugo and Bordo 1999). Is this something to worry about?
The R/Rfree is currently at around 30/32 %. The protein chains are almost complete except for some difficult loops in some chains.
Thanks.
Mohamed
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