When running non-denaturing gel (SDS-gel without reducing agent should work), do you see a dimer? Same question during your sample preparation, if you are performing anything like SEC. If you do have DTT in lysis buffer, that could do. If nothing as such anywhere, honestly no idea...
Cheers, leo
> On 21 Mar 2016, at 15:09, Manjula Ramu <[log in to unmask]> wrote:
>
> I had no reducing agent in my protein buffer as wel as in crystal condition. I have only sodium citrate in the condition.
>
> On Mar 21, 2016 6:54 PM, "CHAVAS Leonard" <[log in to unmask]> wrote:
> DTT perhaps?
>
> Cheers, leo
>
> > On 21 Mar 2016, at 14:04, Pedro Matias <[log in to unmask]> wrote:
> >
> > Does it make sense to consider 2 extra S-atoms bridging the 2 cysteines? What would be the bond distances? What is the meaning of the red e.d. in the 2nd figure?
> >
> > Às 12:44 de 21/03/2016, Eleanor Dodson escreveu:
> >> No idea, but it is a lovely map.
> >>
> >> Are you sure the residue is CYS? You could check the anom map to see if the S give a signal.
> >> Eleanor
> >>
> >> On 20 March 2016 at 04:36, Manjula Ramu <[log in to unmask]> wrote:
> >> Dear community,
> >>
> >> I have got a crystal structure of 16Kda protein, which was solved at 1.9 A. Protein crystallised in dimer form and I could observe a continuous extra density between cysteine of two chains. Distance between two intermolecular cysteine is 7.8 A. Please help me to interpret this extra density. Thanks in advance.
> >> I have attached the screen shots of the map.
> >>
> >>
> >> Thanks and Regards,
> >> Manjula R
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> >>
> >>
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> Leonard Chavas
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Leonard Chavas
-
Synchrotron SOLEIL
Proxima-I
L'Orme des Merisiers
Saint-Aubin - BP 48
91192 Gif-sur-Yvette Cedex
France
-
Phone: +33 169 359 746
Mobile: +33 644 321 614
E-mail: [log in to unmask]
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