Dear all,
I have a general question about cryo-data collection with direct detectors: does it make any difference in terms of high resolution image processing to collect movies in 16 bit vs 32 bit? In principle one can save quite a lot of storage space but I’m not so sure that this procedure will be harmless. Are the high resolution components somehow damaged during the normalisation or particle alignment?
Is there any experience regarding this topic?
Any suggestion will be very appreciated, many thanks in advance.
Edoardo
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Edoardo D'Imprima
PhD Student
Max Planck Institute of Biophysics
Structural Biology Department
Max-von-Laue Straße 3
60438 Frankfurt am Main
Germany
Tel: +49 (0) 69 6303 3015
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