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CCPEM  February 2016

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Subject:

Re: Strategy on combining datasets

From:

HugoMH <[log in to unmask]>

Reply-To:

HugoMH <[log in to unmask]>

Date:

Tue, 16 Feb 2016 20:19:11 +0100

Content-Type:

text/plain

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Dear all,

This is an interesting topic and I have some doubts.

You are talking about rescale one data set but Can you rescale the 
bigger pixel to the smaller? I mean if you have two data sets, the first 
one is at 1 A/pix and the second one at 2 A/pix, Is possible to rescale 
2 A/pix to 1 A/pix.
Images from 2 A/pix might loose information and I guess the information 
should be duplicate or filled the gaps somehow. How does it work?

Thank you

Best

Hugo

-- 
Hugo Muñoz Hernández, PhD student

Centro de Investigaciones Biológicas CIB-CSIC
Consejo Superior de Investigaciones Científicas
C/ Ramiro de Maeztu, 9
28040 Madrid (Spain)

http://www.cib.csic.es/es/grupo.php?idgrupo=47

Tel: +34 91 8373112 ext 4436, Lab.B-47



On 16/02/16 10:09, Sjors Scheres wrote:
> Dear Arko,
> You could rescale one data set to a pixel size as similar as possible as
> the other one, and process the 2 data sets together. In that case be alert
> of classification into the original 2 data sets.
> Alternatively, you could rescale and align the 2 unfil.mrc half-maps of
> one data set on top of the half-maps from the other data set. Then, add
> the half1 maps from both data sets together, as well as the half2 maps.
> You can then perform postprocessing on the 2 summed half-maps to get your
> combined map.
> HTH,
> Sjors
>
>> Dear CCPEM-ers,
>>
>> I would like to have some advice on the optimal way to combine two data
>> sets for single particle reconstruction in relion-should that be performed
>> at the stage of 3D classification (averaging 3D classes?) or after 3D
>> refinement (averaging the 3D volumes obtained in each case)? (in the later
>> case how would one go about calculating 'gold standard' FSC?..Would it be
>> advisable to average the final volumes obtained by separate processing of
>> the two datasets?..Is there a possibility of combining the data sets from
>> scratch? (how to bring the images onto a common scale in that case)?. Any
>> inputs and/or practical advices will be greatly appreciated.
>>
>> Best regards,
>>
>> Arko
>>
>> --
>> *Arka Chakraborty*
>> *ibmb (Institut de Biologia Molecular de Barcelona)*
>> *BARCELONA, SPAIN*
>>
>

-- 
Hugo Muñoz Hernández, PhD student

Centro de Investigaciones Biológicas CIB-CSIC
Consejo Superior de Investigaciones Científicas
C/ Ramiro de Maeztu, 9
28040 Madrid (Spain)

http://www.cib.csic.es/es/grupo.php?idgrupo=47

Tel: +34 91 8373112 ext 4436, Lab.B-47

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