Hi Jianhao,
You could continue this 2D classification with very small angular searches
(or none at all) and then use a mask as wide as the box. Alternatively,
per class (use awk to get a particles.star file for each class you're
interested in form the Class2D/run_data.star) you could use
relion_reconstruct, where you should use --refdim 2 to indicate it's a "2D
reconstruction".
HTH,
Sjors
> Dear Sjors,
>
> I wonder whether it is possible to generate a new stack of average images
> of every classes from Class2D output star file without mask? Because I
> want to do further analysis with it. Thank you!
>
> Best regards,
> Jianhao
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|