Dear Amy,
This is perfectly normal and can happen when the very low-pass filtered
map has little handedness, so covergence can go either way, i.e. into
either hand. Thanks for sharing this. It will help people to be aware that
this might happen.
Best,
Sjors
> Dear Sjors and the rest of ccpem,
> In the Frank lab, we occasionally find that 3D classification introduces a
> mirror-image class among its results. It seems to happen when we filter
> our reference to 60 Angstroms, or lower resolution. This occurs fairly
> rarely and is simple to fix, but we want to report the experience.
>
> Best,
> Amy Jobe
>
>
> --
> PhD candidate, Joachim Frank group
> Department of Biological Sciences
> Columbia University
> 2-221 P & S Black Building
> 650 W. 168th St.
> New York, NY 10032
> Telephone: (315) 879-8192
> E-mail: [log in to unmask]
> Lab website: http://franklab.cpmc.columbia.edu/franklab/
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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