Hi Sameer,
If your resolution is less than 10A, auto-B indeed will not work very well
ad you're stuck with th ad-hoc user-defined one. Also, for such
low-resolution maps, sharpening doesn't usually help much. I would just
use a value that keeps the noise acceptably low, but you yourself are
ultimately the referee on that.
HTH,
Sjors
> Hi,
>
> I'd like to calculate the B-factor for my auto_refined structure in
> Relion, in order to sharpen it. The resolution is currently at 12A, and
> the automatic B-factor estimation doesn't seem to work well for this map.
> I've tried different B-factor values (user-provided B-factor option), and
> while the map does look better, there is obviously a chance I'm over
> sharpening it. I was thus wondering if there is an accurate way I can
> compute the B-factor myself.
>
> Thank you for the help.
>
> Best,
> Sameer
>
> ________________
> Sameer Singh, PhD
> Albert Einstein College of Medicine
> 1300 Morris Park Avenue
> New York, NY - 10461
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|