Hi Laura,
Could you copy the FSC curves from the postprocessing STAR file into an
email to this list?
S
On 01/11/2016 09:18 AM, Laura van Bezouwen wrote:
> Hi,
>
> I was wondering if somebody could help with my mask. I made several masks
> to run on my particle. One mask really helped but another mask not. The
> results were very surprising. I did a 3D classification with the mask and
> took the best class for my 3D refinement. After my 3D refinement I had a
> resolution of 12.6 Å but after postprocessing I ended up with a resolution
> of 23.6 Å. Making a difference mask in Chimera didn't help. Does anybody
> knows what is going on? And how I can improve my results
>
> Thanks for the help,
>
> Best,
>
> Laura
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
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