If you use the EMAN2.1 browser (e2display.py) to display the entire stack (this particular method requires that the entire stack can fit in RAM) middle-click on the stack display window, select "del" mode then click on any particles you want to remove. You can then use "Save" to save the results (excluding the particles marked as deleted) to a new stack file. Alternatively, you can use "Set" mode to generate a list of the particle numbers of the particles you dislike and leave the original stack-file intact.
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Steven Ludtke, Ph.D.
Professor, Dept. of Biochemistry and Mol. Biol. Those who do
Co-Director National Center For Macromolecular Imaging ARE
Baylor College of Medicine The converse
[log in to unmask] -or- [log in to unmask] also applies
http://ncmi.bcm.edu/~stevel
> On Jan 5, 2016, at 2:59 PM, Joshua Lobo <[log in to unmask]> wrote:
>
> Hello CCPEM
>
> I wanted to know if there is a way to interactively view an MRC stack of particles in order to remove bad particles and what would be a reliable way to handle big particle stacks
>
> Any suggestions are welcome
>
> Sincerely
>
> Joshua Lobo
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