Hi Laura,
If you use the automask option of postprocess, you might have to "loosen" the mask by playing around with the --inimask_threshold, --extend_inimask and --width_mask_edge values you use.
Also, if your raw fsc curve is not weird looking, you can try to randomize the phases at a lower resolution, ie lower fsc value (the default value of phase randomizing correspond to the resolution where FSC=0.8), with the option --randomize_at_fsc.
hope it helps!
all the best
celia
Le Lundi 11 Janvier 2016 10:18 CET, Laura van Bezouwen <[log in to unmask]> a écrit:
> Hi,
>
> I was wondering if somebody could help with my mask. I made several masks
> to run on my particle. One mask really helped but another mask not. The
> results were very surprising. I did a 3D classification with the mask and
> took the best class for my 3D refinement. After my 3D refinement I had a
> resolution of 12.6 Å but after postprocessing I ended up with a resolution
> of 23.6 Å. Making a difference mask in Chimera didn't help. Does anybody
> knows what is going on? And how I can improve my results
>
> Thanks for the help,
>
> Best,
>
> Laura
--
--
Dr Célia Plisson-Chastang
Laboratoire de Biologie Moléculaire Eucaryote
UMR CNRS 5099-Université Paul Sabatier Toulouse III
Bâtiment IBCG, 118 Route de Narbonne
31062 Toulouse Cedex, France
Telephone : +33(0)5 61 33 59 50
Fax : +33(0)5 61 33 58 86
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