Dear Xiao Lei,
PHIB denotes the “best phases available” φ-Best
If you are trying to build in an experimentally derived map (eg from SAD or MAD) then there must be a label denoting the phases after density modification, that could be PHIDM for example.
This seems like a map from refinement (of the protein only part?) or from molecular replacement. If thats the case I guess I would choose PHWT (and FWT as the amplitude, for FP).
If you need more info I guess it would be best to explain in more detail what you are trying to do.
Best regards,
Tassos
> On 16 Jan 2016, at 21:02, Xiao Lei <[log in to unmask]> wrote:
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> Hi All,
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> I am using ARP/wARP DNA/RNA version 7.6 in CCP4Interface 7.0 to build DNA, I have two questions:
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> 1. In the GUI, there is a "MTZ in" input, I input the mtz from refmac output, but in the "PHIB" input, it says "unassigned", I choose "list all labels", the labels have FreeR_falg, F, SIGF, FC, PHIC, FC_ALL, PHIC_ALL, FWT, PHWT, DELFWT, PHDELWT, FOM, FC_ALL_LS, PHIC_ALL_LS. I could not find "PHIB" in these labels, and which I should I choose from these labels?
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> 2. If I have a protein-DNA co-crystal (2.7 A resolution), can I just use protein model to do the MR search, refine it in refmac, and use the refmac mtz as input in ARP/wARP DNA/RNA and let the program build DNA?
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> Thanks.
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