Dear community,
the new automated data collection service offered on the ESRF beamline
MASSIF-1 will be taking samples from the 29th January. The service is
available for all types of sample mount and crystals, from screening
initial hits from crystallisation screens through to the collection of
hundreds of data sets from well characterised systems. Essential points:
1. Time is not scheduled but booked on-line when you need it via an
online calendar in consultation with your BAG responsible
2. If you are not part of a BAG you are welcome to apply for rapid
access time here http://www.esrf.eu/MXnon-BAGproposal
3. No restriction on sample type - a rapid X-ray centring routine scans
the full sample mount to locate and centre crystals (crystals <20um are
regularly seen)
4. Data collections can be tailored to each sample (resolution, space
group, beam size, completeness, SAD and more) via the ISPyB diffraction
plan (http://www.esrf.fr/MXPressE)
5. Full results available in real time via ISPyB
6. No need to come to the beamline
7. Time deducted as a function of the number of samples processed
MASSIF-1 has a beam of 100 x 65 um (HxV), that can be defined by
apertures down to 10 um, and has a fixed energy of 12.8 keV with a flux
of ~3 x 10e12 ph/s and a PILATUS3 2M detector.
The service has now been running for over a year and is proving very
popular, we have now processed over 14,000 crystals and 20 structures
have been solved de novo by SAD. Details of the process and the beamline
can be found in 2 recent publications:
Svensson, O., Monaco, S., Popov, A. N., Nurizzo, D. & Bowler, M. W. (2015). The fully automatic characterization and data collection from crystals of biological macromolecules, Acta Cryst. D71, 1757-1767http://dx.doi.org/10.1107/S1399004715011918
Bowler, M.W., Nurizzo, D., et al. (2015). MASSIF-1: A beamline
dedicated to the fully automatic characterisation and data collection
from crystals of biological macromolecules, J. Synchrotron Rad. 22,
1540-1547,http://dx.doi.org/10.1107/S1600577515016604.
For any further questions please don't hesitate to contact me or Didier
Nurizzo ([log in to unmask]). Best wishes, Matthew.
Web:http://www.esrf.eu/MASSIF1
Mail:[log in to unmask]
FAQ:http://www.esrf.eu/MASSIF_FAQ
Twitter:https://twitter.com/ID30_MASSIF1
--
Matthew Bowler
Synchrotron Crystallography Group
European Molecular Biology Laboratory
71 avenue des Martyrs
CS 90181 F-38042 Grenoble
France
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Tel: +33 (0) 4.76.20.76.37
Fax: +33 (0) 4.76.20.71.99
http://www.embl.fr/
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