Dear Tobias,
With a new detector you will attract MANY new users, as you will be able
to do much better structures. The comparison with your current situation
is therefore difficult.
Perhaps a useful statistic: we are currently spending approximately £1000
in computing for every day of successful data collection on our Krios or
Polara microscope with DDD. For running RELION you'd currently need a
cluster with 200-300 cores to run one project at a time.
HTH,
Sjors
> Dear List, Apologies if this question has been asked before. I was
> wondering if there are any publications (or thoughts) of how moving from a
> CCD camera to a direct electron detector will affect computational
> requirements. I’m looking for some evidence (other than back of the
> page calculations based on increased data density) to help to justify
> funding for increasing computational capacity. It’d help to know how
> the groups who have moved to direct detectors have found that computer
> requirements have changed. i.e. I could assume that if we collect X times
> the data then we need X times the capacity, or have users found that the
> improved quality means that they are more selective, so perhaps a lower
> percentage of data collected is processed?
>
> As I mention I’m hoping that someone has written about this before, but
> I’d be happy to hear peoples thoughts on this.
>
>
> Thanks in advance.
>
> TobyS
>
>
> Dr Tobias Starborg
> Senior Experimental Officer: 3DEM
> Wellcome Centre for Cell Matrix Research
> Michael Smith Building
> Manchester
> M13 9PT
> Tel: +44(0)1612755170
> http://www.polara.manchester.ac.uk
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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