Dear Yan,
This would be very difficult to do. So I would not recommend it.
Best,
Sjors
> Hi all,
>
> Thanks for Sjors provided one way to minimize computational costs by using
> down-scaled particles for 2D and 3D classification. However I was thinking
> whether it's possible to achieve this purpose further through the way like
> the following:
>
> After 3D classification using down-scaled particles(in this step the pixel
> size I use is usually 2 A per pixel), I usually perform autorefine on the
> selected down-scaled particles, then switch to original pixel size at 1 A
> per pixel for high resolution refinement. To save computational time,
> could one continue running certain iteration in last refinement by edit
> files and parameters, like the size of needed memory, the size of internal
> maps et al.?
>
> I apologize if it had been raised and discussed.
>
> Thanks,
> Yan
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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