Dear Chuang,
Your input STAR file should also contain a rlnImageName column, with a
referral to the correct image in your stacks. You cannot leave columns
empty in STAR files. If you don't want to pass a certain column, make
sure to remove it from the header as well.
HTH,
Sjors
On 11/19/2015 09:20 PM, Chuang Liu wrote:
> Dear all
>
> I want to do reconstruction using command Relion_reconstruct but with orientation parameter got from other software like Spider.
>
> To do this, I desinged an experiment like this:
>
> Firstly, the dataset used for Relion and Spider was made sure exactly same. Which means that, for a specified particle in Relion dataset, it had same image and same index number as in Spider dataset.
>
> Secondly. Alignment with Spider was run, and each particle's alignment parameters like xshift,yshift,theta,phi,psi were got . After that,the data.star file for relion reconstruction was modified fullfilling columns like voltage,defocus,anglerot,angletilt... .but columns like ClassNumber,LoglikeliContribution were left empty.
>
> Thirdly, the command relion_reconstruct was run with modified data file and particles stack file .
>
> The result was :
>
> "Cannot read file It does not exist
> File: ./src/image.h line: 1040"
>
> I am wondering that is there a way to do this or this is totally impossible?
>
>
> Best regards
> Chuang
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|