Dear all
I want to do reconstruction using command Relion_reconstruct but with orientation parameter got from other software like Spider.
To do this, I desinged an experiment like this:
Firstly, the dataset used for Relion and Spider was made sure exactly same. Which means that, for a specified particle in Relion dataset, it had same image and same index number as in Spider dataset.
Secondly. Alignment with Spider was run, and each particle's alignment parameters like xshift,yshift,theta,phi,psi were got . After that,the data.star file for relion reconstruction was modified fullfilling columns like voltage,defocus,anglerot,angletilt... .but columns like ClassNumber,LoglikeliContribution were left empty.
Thirdly, the command relion_reconstruct was run with modified data file and particles stack file .
The result was :
"Cannot read file It does not exist
File: ./src/image.h line: 1040"
I am wondering that is there a way to do this or this is totally impossible?
Best regards
Chuang
|