Hi Maciej,
In general you have to generate the projections from your 3D and once you
have them you can run a multi-reference alignment to match your 2D Classes
to your projections. One way to get this done is the following. You can
get projections either using IMAGIC (threed-forward command) or Xmipp
(Check this
http://xmipp.cnb.csic.es/twiki/bin/view/Xmipp/CreateProjectionLibrary
,also there was a discussion on the 3DEM about projection matching using
Xmipp if I¡¯m not wrong).
If you¡¯re using IMAGIC, once you have the reprojections you can just run
MRA (multi-ref-align) to align repro and 2D classes. After that you can
sort the 2D classes according to their reference. If you use Tigris it©ös
even faster to display and select the good matches. Maybe you can also use
Eman2 or Xmipp for the multi-reference alignment, but I have never tried.
HTH
Paolo
On 20/11/2015 16:11, "Maciej Jagielnicki" <[log in to unmask]> wrote:
>Hello,
>
>I am in the point of generating figures for my 3D refined map.
>
>I am looking for a way to generate 2D projections (back projections) from
>my final 3D refined map and then matching those 2D projections with 2D
>class averages (i.e. generating 2D projections at certain euler angles
>and then also showing 2d class averages at the same euler angles).
>
>Ideally, a figure would have a set of 1) 2D projections of a 3D refined
>map followed by 2) corresponding 2D class averages followed by 3)
>particles representing each 2D class average.
>
>I would greatly appreciate any suggestions you might have.
>
>Regards,
>Maciej Jagielnicki
>University of Virginia
>
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