Hola Hugo,
If you mean the radius is half the box size (so the diameter equals the
box size) then that's perfectly fine. Shouldn't make a big difference, as
long as you don't cut off your particles with the circle too much.
HTH,
S
> Hi!!!
>
> Relion tutorials and protocols always recommend normalize with a
> reasonable radius for the circle around your particle. After particle
> extraction, I have seen a huge differences using smaller bg_radius. If I
> select a big bg_radius that cover the total box size then 2d
> classification looks nicer.
>
> Could I ask if make sense to normalize with relion_preprocess with a
> same size bg_radius as the box size?
>
> Thank you very much.
>
> Best
>
> Hugo
>
> --
> Hugo Muñoz Hernández, PhD student
>
> Centro de Investigaciones Biológicas CIB-CSIC
> Consejo Superior de Investigaciones Científicas
> C/ Ramiro de Maeztu, 9
> 28040 Madrid (Spain)
>
> http://www.cib.csic.es/es/grupo.php?idgrupo=47
>
> Tel: +34 91 8373112 ext 4436, Lab.B-47
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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