Hi Bettina,
This is probably a singl-precision bug. It will not harm any relion
calculations (relion always ignores the headers), but it will be a pain in
chimera etc. Will fix it for future release.
Thanks for reporting,
Sjors
> Dear All,
>
> I have compiled relion 1.4 with single precision. Now, I often find that
> the origin in the 3D-maps is messed up (see header information below).
> Often the values are very large. Typically that has no further
> implications other than the display in chimera is either messed up or
> reading the map with chimera crashes. I can change the origin with the
> MRC imedit command, then the maps display fine and do not look suspicious.
> Does this error in the origin have any other implications than the
> cosmetic ones mentioned above. Would it be possible to set the origin
> in relion to 0 rather than calculating them? I am confused that the
> values are so big.
>
> Many thanks,
>
> Bettina
>
>
>
>
>
> ------------------------------
> RO image file on unit 1 : run2aa_class1_class001.mrc Size= 1025 K
>
> Number of columns, rows, sections ..... 64 64 64
> Map mode .............................. 2 (32-bit real)
> Start cols, rows, sects, grid x,y,z ... 0 0 0 64 64
> 64
> Pixel spacing (Angstroms).............. 3.056 3.056 3.056
> Cell angles ........................... 90.000 90.000 90.000
> Fast, medium, slow axes ............... X Y Z
> Origin on x,y,z ....................... 0.1587E+27 0.1587E+27
> 0.1587E+27
> Minimum density ....................... -0.30832E-01
> Maximum density ....................... 0.11473
> Mean density .......................... 0.12722E-03
> tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0
> 0.0
> Space group,# extra bytes,idtype,lens . 0 0 0 0
>
> --
> Prof. Bettina Boettcher
> University of Edinburgh
> School of Biological Sciences
> Michael Swann Building (room 334)
> Max Born Crescent
> Edinburgh EH9 3BF
> Phone: + 44 131 6505 699
> http://boettcher.bio.ed.ac.uk/
>
>
> The University of Edinburgh is a charitable body, registered in
> Scotland, with registration number SC005336.
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|