Hi David,
Origin offsets are in pixels in the data.star files, so you should also
change those.
Awk is useful for that.
Also, update the pixel size on the General tab, otherwise the mask
diameter may be off.
HTH,
S
> Hi everyone,
>
> I'd like to add a question on the earlier question on binning the data.
> I have data in a pixelsize of 1.25 A/pixel which I rescaled to a
> pixelframe of 2 A/pixel using the particle extraction panel in Relion.
> This resulted in an enormous gain in speed during classification and
> refinement. During refinement I ran very much against Nyquist frequency.
> So I wanted to go back to the original pixel size. I am not quite sure
> what would be the best way. I cut out all particles in the same way as I
> cut out the rescaled ones just without rescaling and I changed in my final
> data.star from the classification file the rlnDetectorpixelsize back to
> the original one and the rlnImageName to the name of the newly cut
> particles. Is that the intended way?
>
> I actually hope it is, but when I refine the particles after this
> procedure the structure looks considerably worse than in the coarser
> pixelsize and does even refines less well. What could be the reason?
>
> kind regards
>
> David
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|