Diamond and a variety of other SRs have these devices. Check out their webpages for available equipment or contact directly.
Dave
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Phil Evans
Sent: 05 October 2015 09:59
To: ccp4bb
Subject: Re: [ccp4bb] Advice for Structure Determination
Xe at longish wavelength (> 1.5Å), if you can find a pressure device (LURE maybe?)
Phil
> On 5 Oct 2015, at 09:08, Marc Graille <[log in to unmask]> wrote:
>
> Dear Monica,
>
> I had solved couple of structures using anomalous signal for tungsten. I have incubated the proteins with 25-50mM NaWO3 and set-up the crystallization trays.
> If you are lucky, WO3 might interact with your protein as SO4 ions do sometimes and you might be able to get anomalous signals.
> This works fine for nucleic acid binding proteins.
>
> Otherwise, I have also solved a structure of a protein expressed in Pichia using anomalous signal from SeMet. It might be time-consuming and complicated to get labeled protein in Pichia but could be faster than heavy atoms derivatives.
> Here is a link to the publication describing the SeMet labeling procedure.
> http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/26082394
>
> Hope it helps,
>
> Good luck,
>
> Marc
>
>> Le 5 oct. 2015 à 09:38, Monica Mittal <[log in to unmask]> a écrit :
>>
>> Dear all,
>>
>> I need a general advice. I don't have a suitable Model (Lets say only with a similarity of 15%) and no other model to solve the structure by MR. Then i think of anomalous experiments, but soaking with heavy metals is not good for the crystals. Since the protein is being expressed in Pichia cells, growth in Seleno-media is cumbersome. There are no ligands or ions that i can use for anomalous scattering of protein crystals. I am trying to express it in bacteria but have some issues. What is the option that i can choose to solve the structure of such a protein ? Your suggestions are highly recommended.
>>
>> Thanks in advance.
>> Monica
>
> Marc GRAILLE, PhD
> Directeur de recherche CNRS
>
> Laboratoire de Biochimie
> ECOLE POLYTECHNIQUE - UMR7654 CNRS
> 91128 PALAISEAU CEDEX
>
> <Signature-email.jp2>
>
> Phone : +33 (0)1 69 33 48 90 – Fax : +33 (0)1 69 33 49 09
>
> Email: [log in to unmask]
>
> Team: Translation and degradation of eukaryotic mRNAs
> http://bioc.polytechnique.fr/spip.php?rubrique117
> Team supported by the ATIP-Avenir CNRS program
>
>
>
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