Dear Priyamvada,
in this context, note also the following publication which comes with
video tutorials:
Ryan N Jackson et al. (2015) "X-ray structure determination using
low-resolution electron microscopy maps for molecular replacement". Nat.
Protoc. 10 (9), 1275-84.
Tutorial links are in the article e.g.
https://www.youtube.com/watch?v=NH0E2suSiA4
HTH, regards,
Fabio
> Hi Priya,
> You can use the map directly to do MR with MolRep (there is an example on
> its homepage), or convert it to SF first, and the do MR with Phaser, or any
> other program.
>
> To convert it to SF, you need to put it to the center of a unit cell (usu.
> P1), and the unit cell should be big enough (usu. >3x the largest particle
> dimension).
>
> There is a paper by Prof. Yong Xiong, discussing it. And ther are some
> online scripts from other labs.
> Good luck,
> Steve
> On Sunday, October 11, 2015, Acharya, Priyamvada (NIH/VRC) [C] <
> [log in to unmask]> wrote:
>
>> >Apologies for the cross-posting- I posted this on the phenixbb as well,
>> >but posting here as well in case someone has a "non-phenix" suggestion.
>> >
>> >Hi all,
>> >
>> >I want to use an MRC map obtained by low resolution EM reconstruction, as
>> >molecular replacement model in Phaser. I searched the phenixbb archives and
>> >learned that I should use phenix.map_to_structure_factors to convert the
>> >map file to mtz so it can be input into Phaser. When I do that I get an
>> >error "Sorry: Map must have origin at (0,0,0): recenter the map and try
>> >again". I have two questions? 1) What tool should I use to recenter the
>> >.mrc map and 2) Is there a better way to do this?
>> >
>> >Thanks in advance for your replies.
>> >
>> >Best regards,
>> >Priyamvada
--
Dr. rer. nat. Fabio Dall'Antonia
European Molecular Biology Laboratory c/o DESY
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D-22607 Hamburg
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