Dear all
I am facing a problem with 2D classification in relion (1.3 or 1.4).
I have a set of automatically picked particles (10k to 50k) wich contains many "good" but also bad (e.g. from carbon or the hole edge) particles. During 2D classification all particles end up in a single or very few bad classes and all other classes remain empty (black) - no matter how many classes I am asking for.
Has anyone faced a similar situation and knows what knob to turn or which settings to play with? I tried T=1 or 2 so far without improvement.
Many thanks in advance
Christian
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