Dear users,
I am working on a 25kDa protein. The data was processed in
P6122 space group. According to the Matthews coefficient
calculated by "cell content analysis" program in CCP4, it is
a dimer with a probability of 0.98.
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Nmol/asym Matthews Coeff %solvent P(3.53) P(tot)
_____________________________________________________________
1 4.63 73.44 0.02 0.01
2 2.31 46.88 0.97 0.98
3 1.54 20.32 0.00 0.00
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I searched for dimer, but Phaser did not give any solution.
but gave a solution when searched for a monomer.
One round of refinement with the monomer, resulted in an R
and Rfree of 0.26 and 0.32 respectively.
Why PHASER is not giving a dimer? What problem could the data
have in such cases?
Thank you
Regards
Kavya
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