Dear Almudena,
you have to find the NCS operator between the two molecules in ASU and generate new coodinate from the model that you already have the more correctly build. One Other solution maybe if you have already build some equivalent domain in both of the molecules, you can try to superpose molecule A on molecule B according with the equivalent domain as reference.
One last suggestion, have you try to use some autobuild programm, you can activate the NCS research and give your partial model to help. Maybe it will build most of your model automatically.
Hope to help.
Nicolas
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De : CCP4 bulletin board [[log in to unmask]] de la part de Almudena Ponce Salvatierra [[log in to unmask]]
Envoyé : mardi 22 septembre 2015 13:01
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Objet : [ccp4bb] copy fragment and place
Dear all,
I am currently working on a protein of which I have two copies in the AU. I have decent maps for tracing after phasing with TaBr. I have by now traced long stretches of my protein, but the problem is that one of the long stretches is in one of the copies, while the other one is in the second copy. I think the refinement would be much nicer by now if I could combine what I have for each copy.
The question is: Is there a way to copy the residue range I have now traced confidently for one copy, and place it where it should actually be in the other copy?
Thank you and best wishes
Almu
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Almudena Ponce-Salvatierra
Macromolecular crystallography and Nucleic acid chemistry
Max Planck Institute for Biophysical Chemistry
Am Fassberg 11 37077 Göttingen
Germany
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