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ACB-CLIN-CHEM-GEN  September 2015

ACB-CLIN-CHEM-GEN September 2015

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Subject:

Re: Lipaemia index

From:

"Hart, Tanya" <[log in to unmask]>

Reply-To:

Hart, Tanya

Date:

Wed, 2 Sep 2015 15:34:23 +0000

Content-Type:

multipart/mixed

Parts/Attachments:

Parts/Attachments

text/plain (1 lines) , Trig vs L 0-20.jpg (1 lines) , Trig vs L all.jpg (1 lines)

We also use Roche Cobas (8000 & 6000), but the results unfortunately aren't so tidy when it comes to patient samples (attached).



Tanya





Dr Tanya Hart

Clinical Scientist

Department of Clinical Biochemistry

Poole and Royal Bournemouth Hospitals







-----Original Message-----

From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Paul Hamilton

Sent: 02 September 2015 15:54

To: [log in to unmask]

Subject: Re: Lipaemia index



Dear All,



I found a strong correlation between lipaemic index and triglyceride concentration measured on a Roche COBAS 8000 analyser, in a very small experiment using samples spiked with 20% intralipid. See attached chart.



Best wishes,



Paul



Specialty Registrar in Chemical Pathology (Metabolic Medicine) Belfast Health and Social Care Trust Department of Clinical Biochemistry, Kelvin Building, Royal Victoria Hospital Grosvenor Road, Belfast, Northern Ireland, BT12 6BA









--------------------------------------------

On Wed, 2/9/15, Waller, Paul <[log in to unmask]> wrote:



 Subject: Re: Lipaemia index

 To: [log in to unmask]

 Date: Wednesday, 2 September, 2015, 15:34

 

 To add my own unpublished

 observations;

 

 Intralipid

 behaves very differently to endogenous chylomicrons, and can  cause apparent interference in kinetic assays. My unproven  hypothesis was that this was due to detergents/surfactants  in reagents breaking down the lipid vesicles and thus  causing a decrease in absorbance due to turbidity. 

 

 I did not see this phenomenon

 to the same extent in lipaemic patients sera.

 

 

 Paul Waller

 MSc CSci FIBMS FHEA

 Associate Professor in

 Biomedical Science

 Associate Head

 Biomolecular Sciences

 School of Life

 Sciences, Pharmacy and Chemistry

 T 020 8417

 7783 (Direct Dial) / 67783 (Internal)

 E [log in to unmask]

 / Web page Mr Paul Waller

 

 Room PR MB 1019, Penrhyn Road, Kingston upon  Thames, KT1 2EE

 

 Office

 hours by appointment

 

 -----Original Message-----

 From: Clinical biochemistry discussion list  [mailto:[log in to unmask]]

 On Behalf Of [log in to unmask]

 Sent: 02 September 2015 15:24

 To: [log in to unmask]

 Subject: Re: Lipaemia index

 

 Well put, Graham. I was going to reply

 similarly, but I think you describe very well the  variability in chylomicron particle size and their  triglyceride content. As I mentioned previously in this  thread, several publications and my own unpublished  observations show that there is very little correlation  between L (or T)-index and triglyceride concentration in  lipemic specimens.

 

 Note

 that Soha Zouwail mentioned to me off list that she found  good correlation between the triglyceride concentration in  Intralipid and L-index. I have used Intralipid to check for  lipemia (turbidity) interference, but did not look at the  correlation with the L-index. In fact, I think the last time  I did such interference studies was before the specimen  indices era. I think this makes sense though because the  lipid vesicles of Intralipid are likely to be much more  uniform than chylomicrons.

 

 -Jim

 

 -----Original Message-----

 From: Clinical biochemistry discussion list  [mailto:[log in to unmask]]

 On Behalf Of Graham Jones

 Sent: Wednesday,

 September 02, 2015 8:27 AM

 To: [log in to unmask]

 Subject: Re: Lipaemia index

 

 Derreck,

 

 I

 expect not. Rather than having a lot of nice fresh  chylomicrons, all the same size and same triglycerides (TG)  content (and perhaps the same light scattering), I would  expect in any sample we may have a range of difference sizes  and TG content (and light scattering) as they lose TG to  tissues on their way to the liver. Thus the relationship  between TG and light scattering is not very close.

 

 Just my thoughts,

 

 Graham

 ________________________________________

 From: Clinical biochemistry discussion list  <[log in to unmask]>

 on behalf of Mccullough Derreck (UHMB) <[log in to unmask]>

 Sent: Wednesday, 2 September 2015 6:45 PM

 To: [log in to unmask]

 Subject: Re: Lipaemia index

 

 Hi Jim,

  As chylomicrons are

 90% triglyceride would you not expect a good correlation  between triglycerides and turbidity if turbidity is caused  by chylomicrons?

 

 Bw,

 Derreck

 

 -----Original Message-----

 From: Clinical biochemistry discussion list  [mailto:[log in to unmask]]

 On Behalf Of [log in to unmask]

 Sent: 26 August 2015 18:14

 To:

 [log in to unmask]

 Subject: Re: Lipaemia index

 

 Replying to myself here! I agree with Alan that  Sonia's triglyceride vs L- (or T-index) is much higher  than expected. I've had a chance to dig out one of my

 correlations: Triglyceride, mg/dL = 0.88 L-index + 185, R^2  = 0.017. (Sorry for U.S. units, but the key here is the very  low R^2.) My bad that I didn't indicate the  instrument/method on the graph, but it was a Roche  instrument, probably an Integra. Also, earlier I implied  that VLDL, being triglyceride rich, would increase the  L/T-index. VLDL rarely does, but most often it is  chylomicrons that cause the turbidity.

 

 Sonia, you mention that your L-index results  are binned, that is, 1+, 2+, etc. yet your graph has numbers  for the L-index. Does your Architect give you both the  numerical L-index and the binned result?

 

 -Jim

 

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