We also use Roche Cobas (8000 & 6000), but the results unfortunately aren't so tidy when it comes to patient samples (attached).
Tanya
Dr Tanya Hart
Clinical Scientist
Department of Clinical Biochemistry
Poole and Royal Bournemouth Hospitals
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Paul Hamilton
Sent: 02 September 2015 15:54
To: [log in to unmask]
Subject: Re: Lipaemia index
Dear All,
I found a strong correlation between lipaemic index and triglyceride concentration measured on a Roche COBAS 8000 analyser, in a very small experiment using samples spiked with 20% intralipid. See attached chart.
Best wishes,
Paul
Specialty Registrar in Chemical Pathology (Metabolic Medicine) Belfast Health and Social Care Trust Department of Clinical Biochemistry, Kelvin Building, Royal Victoria Hospital Grosvenor Road, Belfast, Northern Ireland, BT12 6BA
--------------------------------------------
On Wed, 2/9/15, Waller, Paul <[log in to unmask]> wrote:
Subject: Re: Lipaemia index
To: [log in to unmask]
Date: Wednesday, 2 September, 2015, 15:34
To add my own unpublished
observations;
Intralipid
behaves very differently to endogenous chylomicrons, and can cause apparent interference in kinetic assays. My unproven hypothesis was that this was due to detergents/surfactants in reagents breaking down the lipid vesicles and thus causing a decrease in absorbance due to turbidity.
I did not see this phenomenon
to the same extent in lipaemic patients sera.
Paul Waller
MSc CSci FIBMS FHEA
Associate Professor in
Biomedical Science
Associate Head
Biomolecular Sciences
School of Life
Sciences, Pharmacy and Chemistry
T 020 8417
7783 (Direct Dial) / 67783 (Internal)
E [log in to unmask]
/ Web page Mr Paul Waller
Room PR MB 1019, Penrhyn Road, Kingston upon Thames, KT1 2EE
Office
hours by appointment
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]]
On Behalf Of [log in to unmask]
Sent: 02 September 2015 15:24
To: [log in to unmask]
Subject: Re: Lipaemia index
Well put, Graham. I was going to reply
similarly, but I think you describe very well the variability in chylomicron particle size and their triglyceride content. As I mentioned previously in this thread, several publications and my own unpublished observations show that there is very little correlation between L (or T)-index and triglyceride concentration in lipemic specimens.
Note
that Soha Zouwail mentioned to me off list that she found good correlation between the triglyceride concentration in Intralipid and L-index. I have used Intralipid to check for lipemia (turbidity) interference, but did not look at the correlation with the L-index. In fact, I think the last time I did such interference studies was before the specimen indices era. I think this makes sense though because the lipid vesicles of Intralipid are likely to be much more uniform than chylomicrons.
-Jim
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]]
On Behalf Of Graham Jones
Sent: Wednesday,
September 02, 2015 8:27 AM
To: [log in to unmask]
Subject: Re: Lipaemia index
Derreck,
I
expect not. Rather than having a lot of nice fresh chylomicrons, all the same size and same triglycerides (TG) content (and perhaps the same light scattering), I would expect in any sample we may have a range of difference sizes and TG content (and light scattering) as they lose TG to tissues on their way to the liver. Thus the relationship between TG and light scattering is not very close.
Just my thoughts,
Graham
________________________________________
From: Clinical biochemistry discussion list <[log in to unmask]>
on behalf of Mccullough Derreck (UHMB) <[log in to unmask]>
Sent: Wednesday, 2 September 2015 6:45 PM
To: [log in to unmask]
Subject: Re: Lipaemia index
Hi Jim,
As chylomicrons are
90% triglyceride would you not expect a good correlation between triglycerides and turbidity if turbidity is caused by chylomicrons?
Bw,
Derreck
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]]
On Behalf Of [log in to unmask]
Sent: 26 August 2015 18:14
To:
[log in to unmask]
Subject: Re: Lipaemia index
Replying to myself here! I agree with Alan that Sonia's triglyceride vs L- (or T-index) is much higher than expected. I've had a chance to dig out one of my
correlations: Triglyceride, mg/dL = 0.88 L-index + 185, R^2 = 0.017. (Sorry for U.S. units, but the key here is the very low R^2.) My bad that I didn't indicate the instrument/method on the graph, but it was a Roche instrument, probably an Integra. Also, earlier I implied that VLDL, being triglyceride rich, would increase the L/T-index. VLDL rarely does, but most often it is chylomicrons that cause the turbidity.
Sonia, you mention that your L-index results are binned, that is, 1+, 2+, etc. yet your graph has numbers for the L-index. Does your Architect give you both the numerical L-index and the binned result?
-Jim
------ACB
discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please
note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=
Green Laboratories Work
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymedicine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e=
List Archives
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0ynXNqWBO9iOXZ39XRyBZ8egc&e=
List Instructions (How to leave etc.)
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=
------ACB discussion
List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages
are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web
Site
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=
Green Laboratories Work
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymedicine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e=
List Archives
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0ynXNqWBO9iOXZ39XRyBZ8egc&e=
List Instructions (How to leave etc.)
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=
------ACB discussion
List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages
are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web
Site
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=
Green Laboratories Work
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymedicine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e=
List Archives
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0ynXNqWBO9iOXZ39XRyBZ8egc&e=
List Instructions (How to leave etc.)
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=
------ACB discussion
List Information--------
This is an open
discussion list for the academic and clinical community working in clinical biochemistry.
Please
note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
Green Laboratories Work
http://www.laboratorymedicine.nhs.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
------ACB discussion List
Information--------
This is an open
discussion list for the academic and clinical community working in clinical biochemistry.
Please
note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
Green Laboratories Work
http://www.laboratorymedicine.nhs.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
Green Laboratories Work
http://www.laboratorymedicine.nhs.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
DISCLAIMER: This email and any files transmitted with it are
confidential and intended solely for the use of the individual,
individuals or entity to whom they are addressed. Any views or opinions
expressed are those of the author and do not necessarily represent the
views of Poole Hospital NHS Foundation Trust unless otherwise explicitly
stated. This email may contain confidential and/or privileged
materials. In the case of confidential and/or privileged materials, any
review, retransmission, dissemination or other use of these materials by
individuals or entities other than the intended recipient may be the
subject of legal action. If you receive an email in error from Poole
Hospital NHS Foundation Trust, please contact the sender and delete the
material from your computer.
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
Green Laboratories Work
http://www.laboratorymedicine.nhs.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
|