Dear Dee,
Throwing away preferred views does not fill the gap in Fourier space of 
the other views. Often, tweeking your sample to get more views (add 
detergents, glow-discharge with amylamine, etc) will lead to a much 
better map.
HTH,
Sjors

PS: awk on the data.star might help you with creating the file you mention.

On 08/29/2015 02:22 AM, Xiaodi Yu wrote:
> Dear all:
>
> I am using Relion/1.4b4 to process my data. I have one data set that shows a very strong preferred view in one direction after 3D Auto-refinement. Right now I plan to throw away some of the images with this view in order to balance the others'. In this case, I should throw away the images based on the 2D classification results. Or I can generate a Preferred_View_Particles.star file based on the Refine3D output files (run1_class001_angdist.bild or run1_data.star files). I don't know which way is better and how people deal with this problem. I personally prefer the second way. But I totally don't know how to generate that file. So any suggestion will be appreciated.
>
> Thank you in advanced and have a good weekend.
>
> Dee

-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres