Dear Nicola,
Please find the response to your question on the Wiki:
http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Minimise_computational_costs
HTH,
Sjors

On 07/03/2015 08:49 AM, Nicola G A Abrescia wrote:
> Dear Sjors,
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> First, I hope you are well.
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> We have done a 3D reconstruction up to 5.6 Ang using binned particles (528 px) in RELION and now we want to use original unbinned particles (1056 px) but without starting from scratch and using the previously determined orientations.
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> Is there an established  protocol for this? Is it possible to use "continue old run" but switching to a re-sized reference and modifying the last    .star file with correct attribution of magnification?
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> Thank you in advance.
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> Best wishes, Nicola
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> -----Original Message-----
> From: Sjors Scheres [mailto:[log in to unmask]]
> Sent: miércoles, 30 de octubre de 2013 13:36
> To: Nicola G A Abrescia
> Subject: Re: FW: access to Krios
>
>
>
> Hi Nicola,
>
> OK, sorry for not including you on the initial message.
>
> S
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> On 10/30/2013 11:14 AM, Nicola G A Abrescia wrote:
>
>> access to Krios
>> Dear Sjors,
>> I have been forwarded the email below by Mikel Valle, my next door
>> colleague.
>> Just to let you know that, as also EM spanish group, I am definitely
>> interested in your proposal.
>> Best wishes, Nicola
>> Dr. Nicola G. A. Abrescia
>> IkerbasqueResearch Professor
>> Structural Biology Unit
>> CIC bioGUNE
>> BizkaiaTechnology Park, Bld 800
>> 48160 Derio - Spain
>> Tel. +34 946 572 523 (ext. 307)
>> http://www.cicbiogune.es/secciones/investigacion/miembros.php?idioma=e
>> n&miembro=163&unidad=5&subgrupo=51
>> ----------------------------------------------------------------------
>> --
>> ----------------------------------------------------------------------
>> --
>> *From:*Sjors Scheres [mailto:[log in to unmask]]
>> *Sent:* Mon 28/10/2013 8:44
>> *To:* [log in to unmask]<mailto:[log in to unmask]>; Mikel Valle; [log in to unmask]<mailto:[log in to unmask]>;
>> [log in to unmask]<mailto:[log in to unmask]>; Carmen San Martin; Jose-Maria Carazo; Oscar
>> Llorca
>> *Cc:* Shaoxia; Lori Passmore; Paula da Fonseca
>> *Subject:* access to Krios
>> Dear Spanish cryoEM groups,
>> As part of our request for funding to MRC 18 months ago, we proposed
>> to allow external cryo-EM groups to have access to the LMB Krios for
>> 30% of the available time until a national facility becomes available.
>> We have already advertised this in the UK, and would now like to
>> extend this invitation to Spanish cryo-EM groups.
>> Within LMB, we have insisted that users have access only after they
>> have demonstrated that their specimens need the increased quality of
>> data that the Krios can provide. Screening for good specimens,
>> optimising freezing conditions and other exploratory usage is done on
>> another microscope, such as T12 or Spirit. We would therefore expect
>> that this preliminary work would also have been carried out in any
>> external users own laboratory.  The idea is for external users to
>> bring their own grids that have been frozen in their home laboratory,
>> and then to take away a hard disk with all the images or movies for
>> computer analysis also back in their own laboratory.
>> The Krios has a Falcon II pre-GIF detector and a Quantum GIF with
>> Gatan
>> K2 post-GIF detector. It is suited to both single particle data
>> collection and cryo-tomography (currently single axis). The single
>> particle data collection can be done (a) manually using the Krios Low
>> Dose system on either the Falcon II or the K2 with energy filtering,
>> or
>> (b) automatically using the FEI EPU system with the Falcon detector.
>> Tomography can be done using SerialEM or Xplore3D; SerialEM works on
>> either the pre-GIF or post-GIF detectors, but Xplore3D works only with
>> the Falcon pre-GIF.
>> All we need from Spanish cryoEM groups wishing to use the Krios is a
>> _brief_ proposal plus a cryo-EM image to demonstrate that they have a
>> specimen that is good enough to produce useful data on the Krios
>> (either for single particle cryoEM or cryotomography).  We are
>> proposing that external users would be allocated one full day (24
>> hours) each week - starting work in the morning and continuing, if
>> necessary, until early next morning.  If there are 10 (UK and Spanish)
>> groups that would like to visit and have suitable specimens, they
>> would expect to have one day for data collection roughly every 10 weeks.
>> Best wishes,  Sjors, Shaoxia, Lori and Paula
>> P.S. We calculate that each day costs £3000 in terms of overall cost,
>> but there will be no charge for access.  Note we are inviting only
>> those UK and Spanish groups that already have their own cryo-EM facilities.
>> Please let us know if you think there are any groups we missed.
>> --
>> Sjors Scheres
>> MRC Laboratory of Molecular Biology
>> Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH,
>> U.K.
>> tel: +44 (0)1223 267061
>> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>> *This e-mail comes from CIC bioGUNE. The e-mail and any files
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>> unauthorised dissemination or copying of this e-mail or its
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>
>
> --
>
> Sjors Scheres
>
> MRC Laboratory of Molecular Biology
>
> Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K.
>
> tel: +44 (0)1223 267061
>
> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>
>
>
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> This e-mail comes from CIC bioGUNE. The e-mail and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any unauthorised dissemination or copying of this e-mail or its attachments, and any use or disclosure of any information contained in them, is strictly prohibited and may be illegal. If you have received this e-mail in error, please notify or telephone + 34 944 06 13 00 and delete it from your system.
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> Please consider the environment before printing this email
>

-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres