Hi Christian,
It often helps to download the relion tutorial data and _exactly_ follow
what happens there. It also contains pre-calculated results for you to
check against.
HTH,
Sjors
> Hi,
>
> I am working on cryo data and managed to get a refined map of around 3.7
> Å resolution using relion (v. 1.3).
>
> Now, I am facing problems getting the particle polishing step to finish.
>
> First of all, I repeatedly get the following error message in the middle
> of the run:
>
> ...
> Postprocessing::run ERROR: FSC curve never drops below randomize_fsc_at
> File: src/postprocessing.cpp line: 594
> ----------------------------------------------------
>
>
> Second, I noticed that during particle extraction from movies, the files
> ...frames_movie_autopick.mrcs are not created, even though the naming of
> the movie files ...frames_movie.mrcs should be correct (with the averaged
> micrographs having file names such as ...frames.mrc
>
> Btw, I also noticed that during processing all particle .star files (e.g.
> particles_autopick.star) are created directly in the project folder, not
> in the dedicated Particles sub-folder. This latter folder remains empty
> for some reason.
>
> It could be that I have problems on multiple levels but I have no clue as
> to where to start looking. Thus, any hint or suggestion will be highly
> appreciated!
>
> Thanks,
>
> Christian
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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