Hi Mike,
This pattern arises from disorder caused by "frustrated overconstraint".
You can find similar pattern in
Welberry, T. R., et al. "Diffuse scattering resulting from macromolecular frustration."
Acta Crystallographica Section B 67.6 (2011): 516-524.
The following review is also interesting, showing that similar physics exists in many different fields:
Keen, David A., and Andrew L. Goodwin.
"The crystallography of correlated disorder." Nature 521.7552 (2015): 303-309.
If you have DIALS installed on your computer, my dials.rs_mapper program
can reconstruct 3D reciprocal space from diffraction images, volume rendering
of which is very illustrative.
Best regards,
Takanori Nakane
On Tue, 28 Jul 2015 21:25:40 +0000 "Keller, Jacob" <[log in to unmask]> wrote:
> This exact type of diffraction pattern was the subject of a long discourse on this BB about five years ago―perhaps a search of the archive will be useful. I think it was eventually described in a paper, with an explanation.
>
> JPK
>
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Mike S
> Sent: Tuesday, July 28, 2015 5:20 PM
> To: [log in to unmask]
> Subject: [ccp4bb] interesting diffraction pattern
>
> Hi ccp4bb members,
>
> I've never seen a diffraction image like this one before - a honeycomb pattern with spots centered in the middle of each hexagon. Any ideas on what might cause this type of pattern? FYI, the protein of interest is hexameric and the crystals display hexagonal morphology.
>
> Thanks for your help.
>
> -Mike
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