But would misindexing affect I/sigI?
And is it necessary to reindex each wedge when translating?
When I do this the indexing program doesn't even know about translation, treats it all as one continuous wedge.
On 07/18/2015 07:26 AM, Harry Powell wrote:
> hi
>
> The Mosflm answer is that if you have inconsistent indexing, Pointless should be your friend here - combining all the MTZ files from Mosflm together in a single run of Pointless should take care of this.
>
> Harry
> --
> Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH
> Chairman of International Union of Crystallography Commission on Crystallographic Computing
> Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
>
> On 18 Jul 2015, at 12:00, Kay Diederichs <[log in to unmask] <mailto:[log in to unmask]>> wrote:
>
>> Christopher,
>>
>> depending on your space group, the problem could be inconsistent indexing, see http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Space_group_determination#dealing_with_alternative_indexing and http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Space_group_determination#Space_groups_as_combinations_of_symmetry_elements
>>
>> HTH,
>>
>> Kay
>>
>> On Sat, 18 Jul 2015 09:34:17 +0100, Christopher <[log in to unmask] <mailto:[log in to unmask]>> wrote:
>>
>>> Hi Community,
>>>
>>> I was hoping to get some advice on a problem I am running into. I have very nice crystals that consistently index well and diffract to about 3.4 Angstrom. Unfortunately, they are very radiation sensitive, thus requiring translation during data collection after acquiring ~2-3 degrees of data. Using either XDS or imosflm, integration and scaling of one "wedge" of data (before translating) results in very nice I/sig values for both the low and high resolution data (+20 and 2 respectively). However, whenever I try to integrate and scale across the entire data range for the crystal, these values drop significantly (ie. 3 and 0.45), eventhough the images being used for integration all have similar intensities and background counts across each resolution shell.
>>>
>>> Does anyone have any tips on how I can process data like this or which parameters I could change that may increase my I/sigI values when integrating the entire data range?
>>>
>>> Thanks so much,
>>> Christopher
|