Hi Sameer,
1. This is not easy at all. You would first need to bring the power
spectra of the 2 maps to something similar. After that you can subtract
the 2 maps in relion_image_handler.
2. Just use image_handler
HTH,
S
>
> Hello,
>
>
>
> I have a couple of queries concerning the generation of density difference
> maps using Relion results.
>
>
>
> 1. I would like to generate a density difference map for a 3D map I
> obtained using Relion and a map that wasn’t created in Relion. I would
> like to first match the intensity scales for the two maps, and wanted to
> know which relion command I can use to do so.
>
>
> 2. Is there a quick way to use Relion to calculate density difference
> maps for classes obtained after a 3D classification run?
>
>
>
> Thank you for the help.
>
>
>
> Best,
>
> Sameer Singh
>
> ________________
> Sameer Singh, PhD
> Albert Einstein College of Medicine
> 1300 Morris Park Avenue
> New York, NY - 10461
> +91-917-848-9319
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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