...and there is a residue missing (159).
B
> It seems your chain ID is different, so it would not connect two
> different chain ID covalently. So please change your chain ID and try to
> connect.
>
>
> Good luck
>
> On Sat, May 23, 2015 at 3:53 PM, Ishan Rathore <[log in to unmask]
> <mailto:[log in to unmask]>> wrote:
>
> Dear All,
>
> I am sorry to disturb you all with this silly question, but this
> problem has been bugging me for quite some time.
>
> Initially 3 residues were missing in the chain, which I had manually
> tried to build, of which 3 residues from the N-terminus which could
> be easily linked by a peptide bond, while the peptide bond could no
> be formed at the C-terminus (I have attached the pic for the same)
> even though the density is quite clear. I have already tried many
> approaches, including linking of two residues in coot and refining,
> but to no avail.
>
>
> Thanks and Regards
> Ishan Rathore
>
>
>
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