Hello everyone
Can anybody suggest me a cryo condition for a crystal obtained in
MIDAS screen of Molecular Dimension:
G1> 0.1MTris8.0G10.1Mpotassium chloride25% v/vSOKALAN®CP7 0.1MHEPES7.0
G2>0.3Mammonium formate20% v/vSOKALAN® CP 5 0.1MHEPES7.0
Crystals are in beautiful cuboid shaped but all sorts of PEG
combinations and Glycerol formulation failed to prevent it from
cracking and dissolving.
Has anybody faced a similar situation as mentioned above and what
precaution was taken to prevent it from cracking or dissolving.
Your suggestions will be of immense help
Thanks in advance
--
Regards
Faisal
School of Life Sciences
JNU
|