Hi Jürgen,
sorry - that's what I get when mailing while boarding ... No, I'd just collect 360 degrees, and if the crystal is still ok, another 360, ... This way one
- obtains high completeness and multiplicity
- can discard frames with "too much" radiation damage
- does not have to worry about the starting point of data collection.
To make the most of the second 360°, you should change some parameter: wavelength, rotation axis (requires a BL with kappa or Prigo), or at least distance (by few percent).
When I read that 1° frames are collected, I just wonder why? Because it used to be done like that in the good old times?
HTH,
Kay
On Fri, 17 Apr 2015 11:55:42 +0000, Jurgen Bosch <[log in to unmask]> wrote:
>Just to clarify, I think what Kay meant with "strategy" is that you don't just shoot at the crystal and collect. You should figure out what is the optimum start and end point of your data collection. Best to be cautious and not immediately go for highest resolution and not fry your crystal. A 4 A complete anomalous data set is better than a partial 3.2A one.
>J?rgen
>
>
>......................
>J?rgen Bosch
>Johns Hopkins University
>Bloomberg School of Public Health
>Department of Biochemistry & Molecular Biology
>Johns Hopkins Malaria Research Institute
>615 North Wolfe Street<x-apple-data-detectors://4>, W8708
>Baltimore, MD 21205<x-apple-data-detectors://5/0>
>Office: +1-410-614-4742<tel:%2B1-410-614-4742>
>Lab: +1-410-614-4894<tel:%2B1-410-614-4894>
>Fax: +1-410-955-2926<tel:%2B1-410-955-2926>
>http://lupo.jhsph.edu<http://lupo.jhsph.edu/>
>
>On Apr 17, 2015, at 06:37, Kay Diederichs <[log in to unmask]<mailto:[log in to unmask]>> wrote:
>
>Hi,
>I'd say using a Pilatus detector in fine-slicing mode and lowdose/high multiplicity will give you better chances to solve the structure. The right strategy makes a difference ...
>Best,
>Kay
>
|