Hi Jian,
Sometimes jobs die due to glitches in the system. You may then miss your
star file. The program has an option --only_make_star that you can use in
this cases: it will only generate the star file, without having to re-run
the whole job.
HTH,
S
> Hi Sjors,
> I met a problem recently when I deal with movie data. I followed manual.
> After ran the CTF estimation, it didn't generate the xxxx_ctf.star file.
> However, I can find all ctf files in the Micrographs folder with for each
> mrc files, for example, ctffind3.com, and ctffind3.log. I used ctffind4 to
> do the ctf correction.
> Is there a way or command to generate the ctf_star file from the results
> in Micrographs folder? Why did it happen? How to correct it?
> The command line I used is shown below
> /opt/relion/1.3/bin/relion_run_ctffind_mpi --i all_AVG.star --o
> all_AVG_ctf.star --ctfWin -1 --CS 2 --HT 300 --AmpCnst 0.1 --XMAG 122058
> --DStep 14 --Box 512 --ResMin 100 --ResMax 7 --dFMin 5000 --dFMax 50000
> --FStep 500 --dAst 0 --ctffind3_exe /opt/ctffind/4.0.8/bin/ctffind
> --omp-num-threads 1 --old-school-input"
> Thanks a lot.
> You have a wonderful weekend.
> Sincerely yours,
> Jian
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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