If you cannot get this running, I would suggest just re-running the ctf
estimation.
HTH, S
On 03/02/2015 02:10 PM, Guan, Jian wrote:
> Hi Sjors,
> Thank you so much for your reply.
> I tried it in command line. However it returned with error:
> Error in textToFloat
> File: src/strings.cpp line:176
>
> Could you please help me with that?
> B.W. Are there any special requirements for the mrc files? Like, 16bit, 32bit or 64bit?
> Thanks,
> Have a good week.
> Jian
>
> -----Original Message-----
> From: Collaborative Computational Project in Electron cryo-Microscopy [mailto:[log in to unmask]] On Behalf Of Sjors Scheres
> Sent: Sunday, March 01, 2015 5:11 AM
> To: [log in to unmask]
> Subject: Re: [ccpem] CTF star file
>
> Hi Jian,
> Sometimes jobs die due to glitches in the system. You may then miss your star file. The program has an option --only_make_star that you can use in this cases: it will only generate the star file, without having to re-run the whole job.
> HTH,
> S
>
>> Hi Sjors,
>> I met a problem recently when I deal with movie data. I followed manual.
>> After ran the CTF estimation, it didn't generate the xxxx_ctf.star file.
>> However, I can find all ctf files in the Micrographs folder with for
>> each mrc files, for example, ctffind3.com, and ctffind3.log. I used
>> ctffind4 to do the ctf correction.
>> Is there a way or command to generate the ctf_star file from the
>> results in Micrographs folder? Why did it happen? How to correct it?
>> The command line I used is shown below
>> /opt/relion/1.3/bin/relion_run_ctffind_mpi --i all_AVG.star --o
>> all_AVG_ctf.star --ctfWin -1 --CS 2 --HT 300 --AmpCnst 0.1 --XMAG
>> 122058 --DStep 14 --Box 512 --ResMin 100 --ResMax 7 --dFMin 5000
>> --dFMax 50000 --FStep 500 --dAst 0 --ctffind3_exe
>> /opt/ctffind/4.0.8/bin/ctffind --omp-num-threads 1 --old-school-input"
>> Thanks a lot.
>> You have a wonderful weekend.
>> Sincerely yours,
>> Jian
>>
>
> --
> Sjors Scheres
> MRC Laboratory of Molecular Biology
> Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K.
> tel: +44 (0)1223 267061
> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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